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New England Biolabs bsshii site
Bsshii Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsshii/BssHII/10__1038_slash_s41467___026___77830___4-430-1-31
Average 96 stars, based on 382 article reviews

bsshii site - by Bioz Stars, 2026-10

96/100 stars

Images

Synthesized:

Article Title: CBP-IDRs regulate acetylation and gene expression
Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

Article Title: CBP-IDRs regulate acetylation and gene expression.
Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

Purification:

Agarose Gel Electrophoresis:

Extraction:

Plasmid Preparation:

Article Title: OT-Curtains: an approach for studying protein interactions with DNA-ends using optical tweezers and confocal fluorescence microscopy
Article Snippet: .. The 22,142 bp 4x poly-BbvCI plasmid (see previous section) is digested with BssHII (New England Biolabs, #R0199S) to linearize the DNA. .. Simultaneously, the plasmid is treated with the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).

Article Title: PD-1 variants having increased PD-L1 affinity
Article Snippet: Specifically, PD-L1 and Fc genes were amplified using primers and Vent polymerase (New England Biolab), respectively, and then assembly PCR was performed using Vent polymerase. .. The prepared gene was treated with BssHII and XbaI (New England Biolab), and the restriction enzyme-treated PD-L1-Fc gene was ligated to a pMAZ vector treated with the same restriction enzyme. ..

Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
Article Snippet: .. The 22 142 bp 4x poly-BbvCI plasmid (see previous section) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. .. Simultaneously, the plasmid is treated with 6 units of the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).

other:

Article Title: CBP-IDRs regulate acetylation and gene expression
Article Snippet: BssHII , NEB , R0199S.

Incubation:

Article Title: Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin.
Article Snippet: .. The sample was then mixed with a 10 μL sample containing 30 units of BssHII (New England Biolabs) diluted in NEB rCutSmart Buffer and incubated at 37◦C (final reaction volume = 55 μL, final reaction buffer = 2.45 mM HEPES pH 7.5/14.5 mM Tris-acetate pH 7.9/1 mM Tris-HCl pH 7.4, 37 mM NaCl/36 mM potassium-acetate, 0.3 mM DTT, 7.6 mM Mg-acetate, 127 μg.mL− 1 recombinant albumin, 10 nM EDTA, and 5.5% (v/v) glycerol). .. 5 μL were removed from the reaction every 5 min for 25 min and quenched with 8 μL of BssHII Stop Solution (10 mM Tris pH 8.0, 0.6% SDS (v/v), 40 mM EDTA, 0.1 mg.mL− 1 Proteinase K (New England Biolabs)).

Recombinant:

DNA Sequencing:

Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy
Article Snippet: After gel extraction, PCR products were cloned into pJET1.2/blunt using the CloneJET PCR Cloning Kit (Thermo Fisher, Waltham, MA, USA). .. After verification by Sanger Sequencing (Eurofins Genomics, Ebersberg, Germany), transgene inserts were excised from pJET1.2/blunt using MluI and BssHII (NEB, Ipswich, MA, USA). .. pcMeVac P-ATU, a MeVac antigenomic vector harboring an additional transcription unit downstream of the P open reading frame, was linearized with MauBI (Thermo Fisher, Waltham, MA, USA).

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